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Chemistry Project Report on Extraction and Determination of Caffeine in Tea Samples Contains in Caffeine
Student of XII-Science (Chemistry)
Submitted to :
Lecturer in Chemistry
Sr. Sec. School
Project Report on "Extraction and Determination of Caffeine Contents in Various Tea Samples"
This project report is on "Extraction and Determination of Caffeine Contents in Various Tea Samples". Caffeine in Tea Samples - Extraction and Determination - Chemistry Experiment, Extraction of Caffeine from Tannic Acid Free Filtrate
Central Board of Secondary Education (CBSE)
Biology project report
Project Report Name : "Extraction and Determination of Caffeine Contents in Various Tea Samples"
Type : Experimental Project Report
"Extraction and Determination of Caffeine Contents in Various Tea Samples"
- Introduction to the topic
- Observations and Expected Errors
Caffeine in Tea Samples - Extraction and Determination - Chemistry Experiment
Most popular drinks in India are “Tea and Coffee” As is the popularity
of these drinks so is the contribution of India in their production. India has
been the leading producer of tea.
Tea grows indeed, fertile & well drained soil. It require warm and moist
climate throughout the year. Frequent showers, well distributed over the year
ensures continuous growth of tender leaves. In India it grows mainly in low
hills of Assam, Hills of Darjeeling and Jalpaiguri, Northern West Bengal and the
hill of Nilgiri etc.
Tea also causes many harmful disease such as Diabetes, problems in nervous
system, decrease in ADN secretion which causes increase in wine secretion and it
has Nicotine which is a Stimulant.
Theory and Chemistry Experiment
Use difference in the solubility of caffeine and tannic acid in water and
chloroform to separate them them from tea leaves.
Caffeine is much more soluble in chloroform than in water. But tannic acid is
extensively soluble in water as well as chloroform.
Extraction of Water Soluble Components from Tea Sample - Step-I
Removal of Tannic Acid from Filtrate - Step-II
- Take about 100ml of distilled water in 250ml conical flask.
- Add 5gm of tea and boil it for 4 to 5 minute. Then cool it and filter.
- Repeat this process again with 100ml of distilled water and residual tea
- The filtrate in each case should be collected.
- Now the wet tea samples may be dried in an over and weighted.
- The filtrate obtained in the first step is now boiled with 2-3 grm.
calcium carbonate to get tannic acid, present in tea extract, frecipitated. On
leaving it for ten minutes ppt. is obtained.
- Filter the solution without disturbing the ppt carefully. The ppt should
be transferred to filter paper only after all the filtrate is removed.
- Tannic acid has been extracted in the form of calcium ternate which on
treatment with hydrochloric acid give tannic acid.
- The filtrate is now free from tannic acid and can be used to extract
Extraction of Caffeine from Tannic Acid Free Filtrate - Step-III
- Take tannic acid filtrate obtained in step 2(ii) and add 100 ml of
chloroform in it in a separating funnel and allow it to stand for 10 minutes.
- Again the same procedure as done in (i)
- Mix if with the previous one.
- From this chloroform extract, chloroform may be distilled or evaporated.
The pale green residue obtained is crude caffeine. Repeat this experiment
with other brands of tea and record your observation in a tabular form.
- Weight of tea used = W1 grm.
- Weight of Caffeine = W2 grm.
Percentage of solute caffeine = W1
Calculations of Double Diamond
Percentage of caffeine = 0.7 / 6 x 100 = 11.6%
Calculations of Lipton Tiger
Percentage of caffeine = 13.3%
Calculations of Taj Mahal
- W1 = 6 gm
- W2 = 0.7 gm
Percentage of caffeine = 11.6%
Calculations of Red Label
Percentage of caffeine = 12%
Name of Brand
%age of caffeine
Experiment Results :
The tea sample of Double Diamond contain highest amount of caffeine and hence :-
- Gives better taste than others.
- Is more harmful for our health.
Precautions and Expected Errors :
- Chloroform should not be smelt directly for a long time as it itself is
- The measurement of weight of tea leaves and all extract should be
- The measurement of weight of tea sample and all extract should be
- The water used should be distilled.
- Apparatus should be clean and dry.
- No water should be added to any extract in between the experiment as it
may get errors in calculation.
- No prolonged heating should be done as water may evaporate in large
quantity along with same volatile solutes.
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